So, for those people only interested in just a fraction of the genome, we are providing a solution that meets their exact needs. For the custom panels in particular, we anticipate it will largely be used by cancer researchers interested in a specific segment(s) of the genetic sequence however, as genetic research escalates exponentially, we expect that it could be used in a much wider array of research - from antibiotics to antibodies to viruses and bacteria.įor instance, a pharmaceutical company doing drug development may only be interested in the kinases, or a physician looking at mutations driving cancer in a patient may only be interested in the 100 or 1,000 genes associated with a particular cancer, or researchers may only be interested in chromosome 18. In general terms, we would sell to customers interested in conducting genetic and genomic research. RM: Can you talk us through some of the applications of your Custom DNA Capture Panels?ĮL: Several of our customers will be presenting at Advances in Genome Biology and Technology who have used our custom panels: Foundation Medicine, which is a cancer research company USAMRIID, who use our probes for viral outbreak research and with whom we worked in partnership with Illumina to develop a custom panviral panel. In addition, with our rapid custom design pipeline, targeted panels can be optimized quickly, allowing the user to scale with minimal time. This flexibility allows the end user the ability to really tailor their enrichment solution to their application without compromise.
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With this enrichment platform, we offer exceptional quality and performance by maximizing capture efficiency with the added value of being able to customize the enrichment solution from panel design to kit configuration. Importantly, we believe the uniformity of our capture means you no longer over sequence your samples - providing future cost savings in terms of increasing the number of samples you can run, increasing the depth of coverage or reducing overall costs. RM: What are the other innovations of your exome and target enrichment solutions announced at AGBT?ĮL: In addition to the double-stranded probes, we've added optimized amplification and NGS QC of the probes, resulting in extreme uniformity of the enriched targets with unparalleled specificity. Being able to tease out real and rare mutations from nucleotide changes due to processing or degradation is critical especially in cancer sequencing. This innovation significantly differentiates our offering by enabling specific capture of both strands of the target, resulting in an increase in the number of unique molecules sequenced and allowing for detection of strand-specific mutations such as deamination events in ancient samples or FFPE. We’ve developed a proprietary method to enable double-stranded probes without compromising target capture. Emily Leproust (EL): Traditionally, probes in double-stranded form have been thought to bind with each other and compete against the formation of target-probe hybrids and in fact, all of the other exome and custom kits in the market today use either single-stranded DNA or RNA. What are the advantages of this approach?ĭr. Ruairi Mackenzie (RM): Your exome and targeting enrichment kits’ probes use double-stranded DNA, which is unique within the industry.
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These include a Human Core Exome Kit, a library preparation and target enrichment kit, and Custom Panels that can be made to individual request. Twist have unveiled a suite of products aimed at enhancing Next Generation Sequencing. Emily Leproust, CEO of Twist Bioscience, to discuss her company's innovative offerings at the recent Advances in Genome Biology and Technology Conference (AGBT) in Orlando.